MelampsoraV1, Main, Exploration, bibRecord, 000008

A comparative analysis of drinking water employing metagenomics.

Identifieur interne : 000008 ( Main/Exploration ); précédent : 000007; suivant : 000009

A comparative analysis of drinking water employing metagenomics.

Auteurs : Kyle D. Brumfield [États-Unis] ; Nur A. Hasan [États-Unis] ; Menu B. Leddy [États-Unis] ; Joseph A. Cotruvo [États-Unis] ; Shah M. Rashed [États-Unis] ; Rita R. Colwell [États-Unis] ; Anwar Huq [États-Unis]

Source :

PloS one [ 1932-6203 ] ; 2020.

RBID : pubmed:32271799

Descripteurs français

KwdFr :
- ADN (génétique), Analyse en composantes principales (MeSH), Bactéries (génétique), Bactéries (pathogénicité), Eau de boisson (microbiologie), Eau de boisson (parasitologie), Gènes bactériens (MeSH), Microbiote (génétique), Métagénomique (MeSH), Numération de colonies microbiennes (MeSH), Virulence (génétique).
MESH :
- génétique : ADN, Bactéries, Microbiote, Virulence.
- microbiologie : Eau de boisson.
- parasitologie : Eau de boisson.
- pathogénicité : Bactéries.
- Analyse en composantes principales, Gènes bactériens, Métagénomique, Numération de colonies microbiennes.

English descriptors

KwdEn :
- Bacteria (genetics), Bacteria (pathogenicity), Colony Count, Microbial (MeSH), DNA (genetics), Drinking Water (microbiology), Drinking Water (parasitology), Genes, Bacterial (MeSH), Metagenomics (MeSH), Microbiota (genetics), Principal Component Analysis (MeSH), Virulence (genetics).
MESH :
- chemical , genetics : DNA.
- genetics : Bacteria, Microbiota, Virulence.
- chemical , microbiology : Drinking Water.
- chemical , parasitology : Drinking Water.
- pathogenicity : Bacteria.
- Colony Count, Microbial, Genes, Bacterial, Metagenomics, Principal Component Analysis.

Abstract

The microbiological content of drinking water traditionally is determined by employing culture-dependent methods that are unable to detect all microorganisms, especially those that are not culturable. High-throughput sequencing now makes it possible to determine the microbiome of drinking water. Thus, the natural microbiota of water and water distribution systems can now be determined more accurately and analyzed in significantly greater detail, providing comprehensive understanding of the microbial community of drinking water applicable to public health. In this study, shotgun metagenomic analysis was performed to determine the microbiological content of drinking water and to provide a preliminary assessment of tap, drinking fountain, sparkling natural mineral, and non-mineral bottled water. Predominant bacterial species detected were members of the phyla Actinobacteria and Proteobacteria, notably the genera Alishewanella, Salmonella, and Propionibacterium in non-carbonated non-mineral bottled water, Methyloversatilis and Methylibium in sparkling natural mineral water, and Mycobacterium and Afipia in tap and drinking fountain water. Fecal indicator bacteria, i.e., Escherichia coli or enterococci, were not detected in any samples examined in this study. Bacteriophages and DNA encoding a few virulence-associated factors were detected but determined to be present only at low abundance. Antibiotic resistance markers were detected only at abundance values below our threshold of confidence. DNA of opportunistic plant and animal pathogens was identified in some samples and these included bacteria (Mycobacterium spp.), protozoa (Acanthamoeba mauritaniensis and Acanthamoeba palestinensis), and fungi (Melampsora pinitorqua and Chryosporium queenslandicum). Archaeal DNA (Candidatus Nitrosoarchaeum) was detected only in sparkling natural mineral water. This preliminary study reports the complete microbiome (bacteria, viruses, fungi, and protists) of selected types of drinking water employing whole-genome high-throughput sequencing and bioinformatics. Investigation into activity and function of the organisms detected is in progress.

DOI: 10.1371/journal.pone.0231210
PubMed: 32271799
PubMed Central: PMC7145143

Affiliations:

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<term>DNA (genetics)</term>
<term>Drinking Water (microbiology)</term>
<term>Drinking Water (parasitology)</term>
<term>Genes, Bacterial (MeSH)</term>
<term>Metagenomics (MeSH)</term>
<term>Microbiota (genetics)</term>
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<term>Eau de boisson (parasitologie)</term>
<term>Gènes bactériens (MeSH)</term>
<term>Microbiote (génétique)</term>
<term>Métagénomique (MeSH)</term>
<term>Numération de colonies microbiennes (MeSH)</term>
<term>Virulence (génétique)</term>
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<front><div type="abstract" xml:lang="en">The microbiological content of drinking water traditionally is determined by employing culture-dependent methods that are unable to detect all microorganisms, especially those that are not culturable. High-throughput sequencing now makes it possible to determine the microbiome of drinking water. Thus, the natural microbiota of water and water distribution systems can now be determined more accurately and analyzed in significantly greater detail, providing comprehensive understanding of the microbial community of drinking water applicable to public health. In this study, shotgun metagenomic analysis was performed to determine the microbiological content of drinking water and to provide a preliminary assessment of tap, drinking fountain, sparkling natural mineral, and non-mineral bottled water. Predominant bacterial species detected were members of the phyla Actinobacteria and Proteobacteria, notably the genera Alishewanella, Salmonella, and Propionibacterium in non-carbonated non-mineral bottled water, Methyloversatilis and Methylibium in sparkling natural mineral water, and Mycobacterium and Afipia in tap and drinking fountain water. Fecal indicator bacteria, i.e., Escherichia coli or enterococci, were not detected in any samples examined in this study. Bacteriophages and DNA encoding a few virulence-associated factors were detected but determined to be present only at low abundance. Antibiotic resistance markers were detected only at abundance values below our threshold of confidence. DNA of opportunistic plant and animal pathogens was identified in some samples and these included bacteria (Mycobacterium spp.), protozoa (Acanthamoeba mauritaniensis and Acanthamoeba palestinensis), and fungi (Melampsora pinitorqua and Chryosporium queenslandicum). Archaeal DNA (Candidatus Nitrosoarchaeum) was detected only in sparkling natural mineral water. This preliminary study reports the complete microbiome (bacteria, viruses, fungi, and protists) of selected types of drinking water employing whole-genome high-throughput sequencing and bioinformatics. Investigation into activity and function of the organisms detected is in progress.</div>
</front>
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<Abstract><AbstractText>The microbiological content of drinking water traditionally is determined by employing culture-dependent methods that are unable to detect all microorganisms, especially those that are not culturable. High-throughput sequencing now makes it possible to determine the microbiome of drinking water. Thus, the natural microbiota of water and water distribution systems can now be determined more accurately and analyzed in significantly greater detail, providing comprehensive understanding of the microbial community of drinking water applicable to public health. In this study, shotgun metagenomic analysis was performed to determine the microbiological content of drinking water and to provide a preliminary assessment of tap, drinking fountain, sparkling natural mineral, and non-mineral bottled water. Predominant bacterial species detected were members of the phyla Actinobacteria and Proteobacteria, notably the genera Alishewanella, Salmonella, and Propionibacterium in non-carbonated non-mineral bottled water, Methyloversatilis and Methylibium in sparkling natural mineral water, and Mycobacterium and Afipia in tap and drinking fountain water. Fecal indicator bacteria, i.e., Escherichia coli or enterococci, were not detected in any samples examined in this study. Bacteriophages and DNA encoding a few virulence-associated factors were detected but determined to be present only at low abundance. Antibiotic resistance markers were detected only at abundance values below our threshold of confidence. DNA of opportunistic plant and animal pathogens was identified in some samples and these included bacteria (Mycobacterium spp.), protozoa (Acanthamoeba mauritaniensis and Acanthamoeba palestinensis), and fungi (Melampsora pinitorqua and Chryosporium queenslandicum). Archaeal DNA (Candidatus Nitrosoarchaeum) was detected only in sparkling natural mineral water. This preliminary study reports the complete microbiome (bacteria, viruses, fungi, and protists) of selected types of drinking water employing whole-genome high-throughput sequencing and bioinformatics. Investigation into activity and function of the organisms detected is in progress.</AbstractText>
</Abstract>
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<AffiliationInfo><Affiliation>University of Maryland Institute for Advanced Computer Studies, University of Maryland, College Park, MD, United States of America.</Affiliation>
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<CoiStatement>The authors declare the follow potential conflicts of interest with respect to the research, authorship and/or publication of this article: RRC is the founder of CosmosID, Inc., Rockville, MD and also a Professor at the University of Maryland; NAH and SMR are employees of CosmosID, Inc., Rockville, MD; JAC is the founder of Joseph Cotruvo and Associates LLC, Washington, DC; MBL is an employee of Essential Environmental and Engineering Systems, Huntington Beach, CA. The specific roles of these authors are articulated in the ‘author contributions’ section. This does not alter our adherence to PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors (https://journals.plos.org/plosone/s/competing-interests).</CoiStatement>
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<name sortKey="Rashed, Shah M" sort="Rashed, Shah M" uniqKey="Rashed S" first="Shah M" last="Rashed">Shah M. Rashed</name>
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   |clé=     pubmed:32271799
   |texte=   A comparative analysis of drinking water employing metagenomics.
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Serveur d'exploration Melampsora

A comparative analysis of drinking water employing metagenomics.

A comparative analysis of drinking water employing metagenomics.

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